Mode of action clustering of chemicals and environmental samples on the bases of bacterial stress gene inductions.

نویسندگان

  • Freddy Dardenne
  • Stefan Van Dongen
  • Ingrid Nobels
  • Roel Smolders
  • Wim De Coen
  • Ronny Blust
چکیده

Over the years, environment and the human population have seen an increasing exposure to both existing and newly developed chemicals. It is generally accepted that at least some of those are toxic, albeit as pure compound or in combination with others. In response to a growing public awareness and scientific data, the new European chemicals legislation (Registration, Evaluation and Authorization of Chemicals) is under implementation at the moment. As a consequence, during the coming years about 30,000 chemicals have to be assessed on their potential hazard for man and biota. Part of this assessment will be done using existing and new in vitro tests offering insight into the toxicity of chemicals and into their toxicological mode of action. This study presents data on a battery of 14 bacterial reporter gene assay allowing mode of action determination and statistical grouping of chemicals based on their induction profile. Gene induction results are used to group reference chemicals in a statistical cascade employing hierarchical tree and k-means clustering for initial grouping. Both complementary, yet mathematically different, algorithms are consequently confirmed by principal component analysis (PCA). The gene induction profiles of an environmental extract with documented in vivo effects and a chemical with limited toxicological are data available and projected in the PCA vector space. The projection allows correct mode of action grouping and indicates that effect predictions based on the known toxicological effects of the reference compounds can be made.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Microarray analysis of gene expression patterns in Arabidopsis seedlings under trehalose, sucrose and sorbitol treatment

Trehalose is the non-reducing alpha-alpha-1, 1-linked glucose disaccharide. The biosynthesisprecursor of trehalose, trehalose-6-phosphate (T6P), is essential for plant development, growth,carbon utilization and alters photosynthetic capacity but its mode of action is not understood. In thecurrent research, 6 days old seedlings of Arabidopsis thaliana (Columbia ecotype) were grown inliquid cultu...

متن کامل

Gene Effects for Agronomic Traits in Safflower (Carthamus tinctorius L.) under Drought Stress

The mode of agronomic traits inheritance was investigated in safflower (Carthamus tinctorius L.) in drought stress as a new report. Five generation including P1, P2, F1, F2 and F3 that derived from the cross of IL.111 (drought sensitive) ×Mex.22-191 (drought tolerance) were used in a randomized complete block design with two replic...

متن کامل

Secreted Chemicals From Probiotic Bacteria Potentiate Th1 Pattern of Immune Cells and Apoptosis Induction in Breast Cancer and Gastric Adenocarcinoma Cell Lines

Cancer is one of the main causes of mortality. Therapeutic importance in some diseases, especially cancers has led to considering the anti-cancer effects of probiotics more than ever. Probiotics have stimulatory effects on the immunity and suppressor effects on cancer activity. Various studies report that probiotic bacteria have cytotoxicity effects on different types of solid tumors. In this r...

متن کامل

Serological Classification and Comparison of Cell Surface Hydrophobicity and Biofilm and Proteases Formation between the Clinical and Environmental Isolates of Pseudomonas Aeruoginosa

Background & Aims: Pseudomonas aeruoginosa is an opportunistic pathogen and an important cause of nosocomial infections. Different factors are involved in the pathogenicity of this bacterium. This study was performed to compare some factors associated with the virulence of clinical and environmental isolates of P. aeruoginosa. Methods: The present study was performed on 25 environmental isolate...

متن کامل

کاربرد SPME به عنوان یک نمونه بردار غیر فعال در ارزیابی تماس های استنشاقی-مطالعه موردی بر روی 2-کلروفنول

  Background and aims: Halogeneated hydrocarbons are among the most important classes of toxic chemicals considering environmental and occupational health which can be quantified by several methods. This study examined the applicability of solid phase microextraction for sampling and quantifying of 2-Chlorophenol in the air.   Methods:A dynamic standard atmosphere generation system was construc...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Toxicological sciences : an official journal of the Society of Toxicology

دوره 101 2  شماره 

صفحات  -

تاریخ انتشار 2008